A simple and effective cryopreservation protocol for the industrially important and model organism, Euglena gracilis

STAR Protoc. 2021 Dec 15;3(1):101043. doi: 10.1016/j.xpro.2021.101043. eCollection 2022 Mar 18.

ABSTRACT

Euglena gracilis is a source of high-value natural products. A major factor affecting consistent production of Euglena biomass is strain stability. Cryopreservation is a leading strategy for cell-line storage that helps ensure process reproducibility. We developed a simple cryopreservation protocol for heterotrophically cultured Euglena that enables the recovery of cells after 1 year with a cell viability of ≅80%. This protocol is suitable for labs interested in the long-term preservation of heterotrophic cultures of Euglena and related species.

PMID:34977684 | PMC:PMC8686060 | DOI:10.1016/j.xpro.2021.101043

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