MethodsX. 2022 Dec 16;10:101976. doi: 10.1016/j.mex.2022.101976. eCollection 2023.
Hydrogen is generally considered as an ideal non-polluting future energy carrier because it releases energy and water as a byproduct on combustion. Besides, hydrogen possesses the highest energy density on mass basis compared to any other fuel. However, hydrogen production in a sustainable and environmentally friendly way still remains a challenge. Recently, biohydrogen production from green microalgae has gained significant attention due to availability of the feedstock, which are environmentally friendly and renewable. Biohydrogen production from photosynthetic microalgae is attractive, however in the current context, it has a low yield, and an optimization of the affecting parameters including algae concentration, light intensity, culture medium, etc. is critical. In this study, biohydrogen was produced in laboratory from Euglena acus microalgae as it was locally available in Bangladesh.•The effect of two different culture mediums (i.e. sulfur-rich and sulfur-deprived TAP mediums) for microalgae cultivation and biohydrogen yield were studied.•Depending on the concentration of microalgae (50% and 75% by weight) in the medium solution ∼3 ml to 5 ml biohydrogen was obtained.