Earthquakes occur frequently in fragile alpine grassland areas on the Qinghai-Tibet Plateau (QTP), but few studies have evaluated the impacts of seismo-fault of earthquake on alpine grassland vegetation diversity. In this study, we conducted a field survey of plant communities of alpine grassland along the fault zone in the 7.4 Maduo earthquake occurred on 22 May 2021. Surrounding grassland habitat far from the seismo-fault of earthquake was selected as the control. Plant community metrics around and far from seismic rupture were studied. The results showed that plant community metrics were negatively affected by seismo-fault of earthquake. Species composition around seismo-fault was being shifted from sedges-dominant into forbs-dominant. In addition, the diversity and aboveground biomass were significantly decreased around seismo-fault compared with the control. Our findings highlighted that earthquakes can cause species loss and plant community shift and finally lead to productivity reduction of alpine grassland. Additionally, forbs may be more competitive than other functional groups after the earthquake.