Establishment of an in vitro thrombogenicity test system with cyclic olefin copolymer substrate for endothelial layer formation

MRS Commun. 2021 Sep 7:1-9. doi: 10.1557/s43579-021-00072-6. Online ahead of print.

ABSTRACT

In vitro thrombogenicity test systems require co-cultivation of endothelial cells and platelets under blood flow-like conditions. Here, a commercially available perfusion system is explored using plasma-treated cyclic olefin copolymer (COC) as a substrate for the endothelial cell layer. COC was characterized prior to endothelialization and co-cultivation with platelets under static or flow conditions. COC exhibits a low roughness and a moderate hydrophilicity. Flow promoted endothelial cell growth and prevented platelet adherence. These findings show the suitability of COC as substrate and the importance of blood flow-like conditions for the assessment of the thrombogenic risk of drugs or cardiovascular implant materials.

SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1557/s43579-021-00072-6.

PMID:34513262 | PMC:PMC8422954 | DOI:10.1557/s43579-021-00072-6

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