Sesame is considered as the queen of oil seeds owing to its high oil content of about 56-62% and good quality oil. Sesame oil alone or in combination with other vegetable oils can yield good quality biodiesel. Sesame biodiesel blends up to 20% yields fuel efficiency and power output on par with mineral diesel but superior in environmental performance. Though biodiesel from edible oil is highly criticized, the demand for renewable energy compels the development of high-performance sesame plants. Triacylglycerol synthesis in flowering plants follows an acyl CoA-dependent and independent manner. This study envisages transgenic approaches to enhance oil production in sesame biomass. The genes of choice for oil enhancement includes DGAT1, PDAT1, FAD3 and cytochrome b5F. Diacylglycerol acyltransferase (DGAT) and phospholipid diacylglycerol acyltransferases (PDAT) are key enzymes in TAG synthesis. Fatty acid desaturases (FAD) has the ability to enhance specific fatty acids, whereas cytochrome b5 genes augment the process by donating electrons. A combination of the above categories of genes which performed well in terms of oil content in the yeast expression system from our earlier studies is used in Agrobacterium-mediated sesame transformation experiments to evaluate the biodiesel potential of transgenic sesame plants. The transgenic construct with PDAT1 and FAD3 combination yielded a 10% increase in TAG content. The possibility of transgenic sesame as a biodiesel plant is discussed.