Novel biosynthesis of MnO NPs using Mycoendophyte: industrial bioprocessing strategies and scaling-up production with its evaluation as anti-phytopathogenic agents

Sci Rep. 2023 Feb 4;13(1):2052. doi: 10.1038/s41598-023-28749-z.


This report provides the first description of the myco-synthesis of rod-shaped MnO NPs with an average crystallite size of ~ 35 nm, employing extracellular bioactive metabolites of endophytic Trichoderma virens strain EG92 as capping/reducing agents and MnCl2·4H2O as a parent component. The wheat bran medium was chosen to grow endophytic strain EG92, which produced a variety of bioactive metabolites in extracellular fraction, which increases the yield of MnO NPs to 9.53 g/l. The whole medium and fungal growth conditions that influenced biomass generation were optimized as successive statistical optimization approaches (Plackett-Burman and Box-Behnken designs). The production improvements were achieved at pH 5.5, WBE (35%), and inoculum size (10%), which increased Xmax to twelve-folds (89.63 g/l); thereby, Pmax increased to eight-folds (82.93 g/l). After 162 h, Xmax (145.63 g/l) and Pmax (99.52 g/l) on the side of µmax and YX/S were determined as 0.084 and 7.65, respectively. Via Taguchi experimental design, fungus-fabricated MnO NPs reaction was improved by adding 0.25 M of MnCl2·4H2O to 100% of fungal extract (reducing/capping agents) and adjusting the reaction pH adjusted to ~ 5. This reaction was incubated at 60 °C for 5 h before adding 20% fungal extract (stabilizing agent). Also, Pmax was raised 40-fold (395.36 g/l) over the BC. Our myco-synthesized MnO NPs exhibit faster and more precise antagonistic actions against phytopathogenic bacteria than fungi; they could be employed as an alternative and promised nano-bio-pesticide to manage a variety of different types of disease-pathogens in the future.

PMID:36739323 | PMC:PMC9899258 | DOI:10.1038/s41598-023-28749-z


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