Size fractionation with 2000 and 1000 μm screens is used by the Institute of Marine Research in Norway in routine monitoring of zooplankton biomass. This study examines the separation of taxa by this procedure. For copepods and cladocerans, the fractionation separates individuals according to their size in a consistent and predictable manner. Individuals up to 0.4 mm in width are contained in the small fraction (<1 mm). From width 0.4 to 0.8 mm, there is a progressive shift from the small to the medium fraction (1-2 mm). From about 0.8 mm width, individuals start to be contained in the large fraction (>2 mm). For Calanus finmarchicus, young copepodites CI-CIII are contained in the small fraction, while the older stages CV and adults are contained in the medium fraction. Small copepods (Oithona, Oncaea, Microcalanus, Pseudocalanus) are contained in the small fraction, as are most appendicularians and meroplanktonic invertebrate larvae. The large fraction includes large copepods, larger individuals of chaetognaths, krill and amphipods. The consistency of separation of taxa by size will help to interpret and improve the ecological relevance of results on size-fractioned zooplankton biomass in the Barents Sea as well as other high-latitude areas.