Technique for Darkening of Extracted Teeth Simulating Pulpal Necrosis Discoloration

Clin Cosmet Investig Dent. 2022 Apr 28;14:103-112. doi: 10.2147/CCIDE.S361230. eCollection 2022.


BACKGROUND: The use of discolored teeth is required to test whitening products, and it is difficult to obtain them, given their scarcity.

OBJECTIVE: To present a technique for in vitro darkening of extracted teeth simulating pulpal necrosis discoloration.

MATERIALS AND METHODS: Hemolysates I and II from human blood were subjected or not to laser irradiation (442 nm) for 1 h. The concentration of oxyhemoglobin (O2Hb) was analyzed by ultraviolet/visible spectroscopy, and the conversion of O2Hb to methemoglobin (MetHb) by transmission spectroscopy was assessed immediately and after 3 and 40 days. For darkening evaluation, bovine incisors were divided into two groups (n = 25), and their pulp chambers were filled with hemolysate solution II (HSII) and hemolysate II solution + laser (HSII+L). After storage in artificial saliva for 40 days at 37°C, color changes were measured by a colorimeter and ΔE was compared with the NBS parameters. Data were analyzed using a mixed linear model (α=5%).

RESULTS: HSII+L presented the lowest O2Hb and higher MetHb. The conversion of O2Hb to MetHb in HSII+L was 42% higher than in HSII. Both groups were effective in darkening the teeth, according to the NBS. Darkening stabilized from day 35. HSII promoted a marked color difference.

CONCLUSION: The proposed technique was effective in darkening the extracted teeth simulating necrosis discoloration for in vitro models.

PMID:35509813 | PMC:PMC9059876 | DOI:10.2147/CCIDE.S361230


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